Bot1-1 mutant phenotype.

(a) Seven-day-old light-grown wild-type (left) and bot1-1 (right) seedlings.

(b) Four-week-old wild-type (left) and bot1-1 (right) plants grown in a greenhouse.

(c) Adult plant of wild-type (left) and various bot1 alleles plants: from second left to right, bot1-1, bot1-3, bot 1-4, bot 1-6, bot 1-5, bot 1-2 (bot 1-6 was not characterized in this work). The plants were grown for 6 weeks in the greenhouse.Cellular defects in bot1-1.

(d) SEM (left) and transverse sections (right) of hypocotyls of 7-day-old light-grown wild-type (top) and bot1-1 (bottom) seedlings. Scale bar: 100 µm.

(e) SEM (left) and transverse sections (right) of hypocotyls of 7-day-old dark-grown wild-type (top) and bot1-1 (bottom) seedlings. Scale bar: 100 µm.Flowerstem anatomy.

(f) Hand-cut transverse sections through mature flower stems of wild-type (left) and bot1-1 (right) 6-week-old plants: (top) general structure of the flower stem (bottom) detail of the vascular bundles. Scale bars: 500 µm.