Morphology of rsw3.

(a) Root system of a seedling showing that lateral roots extend some distance before swelling and stopping elongation. Plants grown 5 days at 21°C and 6 days at 30°C. Scale bar = 2 mm.

(b) Continued root growth gives a dense, highly branched root system and a dense mass of very small leaves on plant grown for 21 days at 30°C. Scale bar = 5 mm.

(c) Hypocotyls grown in the dark for 3 days at 21°C and 3 days at 30°C. From the left: wild-type, rsw1-1, rsw2-1, rsw3-1, rsw1-1rsw2-1, rsw1-1rsw3-1. The rsw3 effect on the hypocotyl is weak compared to that of the other single mutants and rsw1-1rsw3 is weaker that rsw1-1rsw2-1. Scale bar = 5 mm.

(d) Light micrograph of rsw3 grown on agar for 35 days at 30°C. Tiny inflorescences with flower buds of near normal size (top right and bottom left) emerge from several of the rosettes. Scale bar = 5 mm.

(e) Scanning electron micrograph of rsw3 plant grown for 21 days at 30°C and showing the presence of multiple rosettes. Scale bar = 1 mm.

(f) Detail of the ringed area in (e) showing the very complex arrangement of the minute leaves, many of which carry trichomes of approximately normal size and morphology. Scale bar = 200 m.

(g) Scanning electron micrograph of the surface of a wild-type leaf on a plant grown for 10 days at 30°C. Note the clearly defined cell boundaries, stomata and trichomes.

(h) The surface of a rsw3 leaf showing much less clear outlines to the pavement cells, an apparently collapsed trichome (CT) on top of its ring of subsidiary cells and many stomata with their guard cells protruding above the leaf surface. Scale bar for (g) and (h) = 100 m.