![]() |
Light micrographs showing morphology and histology
of rsw4 and rsw7 roots. (A-C) Paired images of a roots
grown under (left-hand image) the permissive treatment (8 days at 19°C)
and under (right-hand image) the restrictive treatment (6 days at 19°C
followed by 2 days at 30°C). (A) Wild type; (B) rsw4; (C)
rsw7. Bar: 100 µm. (D-L) Cross sections of roots within
the region of maximal diameter stained with PAS to show cell walls: (D-F)
Wild type; (G-I) rsw4; (J-L) rsw7. The left hand column
(D,G,J) shows the anatomy of plants grown under the permissive treatment,
the center column (E,H,K) the restrictive treatment, and the right-hand
column (F,I,L) the permissive treatment supplemented with 10 µg/ml
aphidicolin for the 2 days at 30°C (wt and rsw7) or for only
the second day (rsw4). Bar: 50 µm.
|