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1. Pathways of substrate generation
The synthases and glycosyl transferases involved in the synthesis of cell-wall
polysaccharides use nucleotide-sugars exclusively as activated substrates.
The de novo synthesis of cell-wall monosaccharides occurs via the nucleotide-sugar
When hydrolyzed from polysaccharides, several monosaccharides made via this pathway are salvaged into the nucleotide-sugar pools by C-1 kinases and nucleotide-diphosphate-pyrophosphorylases. Genes of several epimerases and C-1 kinases have been identified but their specific functions have not been confirmed.
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