I. Isolation of Cell Walls

II. Cellulose Determination; Total Sugar and Uronic Acids

III. Fractionation of Cell Wall Polysaccharides

IV. Methyl Esterification of Uronic Acids

V. Monosaccharide Composition

VI. Linkage (methylation) Analysis

VII. Fourier Transform Infrared (FTIR)

VIII. Protocol for the screening of the UniformMu maize population with near infrared reflectance spectroscopy


These techniques have been adapted from many classical ‘recipes’ that do not bear formal literature citations. However, some of the primary methods literature is included where appropriate. Current literature is also recommendation for citation if significant deviations or improvements to the classic methods have been made. Most researchers have adapted these standard methods further for their own specific applications. For several of these methods and some alternatives, see the manual by Stephen C. Fry (1988) The Growing Cell Wall: Chemical and Metabolic Analysis. Harlow, Essex, England: Longman Scientific & Technical, and subsequently, New York: Wiley.